Click-&-Go[R] Plus 594 OPP
Product Sizes
1 kit
CCT-1495-1KIT
About this Product
- SKU:
- CCT-1495
- Application:
- Conjugation/Labeling
- Extra Details:
- Click-&-Go® Plus 594 OPP Protein Synthesis Assay Kit enables fast and sensitive imaging of nascent proteins in complete, methionine-containing media. OPP (O-propargyl-puromycin) is an analog of puromycin that contains a terminal alkyne group, enters the acceptor site of ribosomes and incorporates into nascent polypeptide chains. Unlike HPG or AHA labeling reagents OPP is not an amino acid analog; thus, OPP can be added directly to cells in complete media (i.e., methionine-containing) or used to detect in vivo protein synthesis. In the next step, OPP-labeled nascent proteins are imaged through a chemoselective ligation, or "click" reaction, between the red fluorescent AZDye 594 Azide Plus (Alexa Fluor 594 ® equivalent) and the OPP-alkyne allowing the modified proteins to be detected by imaged-based analysis. The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm x 18 mm coverslips using 1 mL of reaction buffer per test.
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:Click-Go-Plus-594-OPP
