Click-&-Go[R] Plus 555 OPP
Product Sizes
1 kit
CCT-1494-1KIT
About this Product
- SKU:
- CCT-1494
- Application:
- Conjugation/Labeling
- Extra Details:
- Click-&-Go® Plus 555 OPP Protein Synthesis Assay Kit enables sensitive, and non-radioactive chemical method to image and affinity-purify nascent proteins in cells and in animals, based on an alkyne analog of puromycin, O-propargyl-puromycin (OPP). OPP is an analog of puromycin that contains a terminal alkyne group, enters the acceptor site of ribosomes and incorporates into nascent polypeptide chains. OPP forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized through copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms. This strategy should have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo.
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Manufacturer's Data Sheet:Click-Go-Plus-555-OPP
