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Proteins and Peptides

Click-&-Go[R] Dde Protein Enrichment Kit

Product Sizes
1 kit
CCT-1153-1KIT
About this Product
SKU:
CCT-1153
Application:
Conjugation/Labeling
Extra Details:
Click-&-Go® Dde Protein Enrichment Kit is an efficient, biotin/streptavidin-free tool for capturing alkyne-modified biomolecules on a cleavable agarose resin via click reaction and its subsequent release under mild conditions. Alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the cleavable azide agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with high stringency virtually eliminating any non-specifically bound proteins. Treatment with 2% hydrazine yields a highly enriched population of nascent proteins. Captured proteins can be protease digested to yield a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores