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Red Fluorescent SR-FLICA[R] Caspase-8 (LETD) Assay Kit

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£286.00

SKU:: 9149
Anwendung:: Flow Cytometry
Physischer Zustand:: Cell Culture
Aufreinigung:: Jurkat cells were grown in suspension to 4 x 105 cells/mL, then divided into two separate TC-flasks. One population, "Non-Induced", received a DMSO vehicle control (A). The other population, "Induced", was spiked with 1 uM staurosporine (B). Cells were incubated for 4 hours at 37°C, then stained with ICT's red SR-FLICA caspase-8 inhibitor, SR-LETD-FMK (kit catalog #9150) for 1 hour at 37°C. After labeling, samples were washed three times and slides were prepared. Fluorescence images were acquired using a Nikon Eclipse 90i microscope equipped with a Hamamatsu Flash 4.0 camera. In the treated sample, cells appear bright red, indicating a high level of caspase-8 activity (B, Induced, right). In the non-induced sample, few red positive cells are visible, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).
Lagerbedingungen:: 2-8[o]C
Hersteller:: ImmunoChemistry Technologies
Immunogen:: Caspase 8
Weitere Details:: This in vitro assay employs the red fluorescent inhibitor probe SR-LETD-FMK to label active caspase-8 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT