9105
TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay
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£366.00
- SKU:
- 9105
- Anwendung:
- Flow Cytometry
- Physischer Zustand:
- Cell Culture
- Aufreinigung:
- Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and stained with ICT's potentiometric dye TMRM. Cells were analyzed using a Nikon E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence.
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- ImmunoChemistry Technologies
- Immunogen:
- Mitochondrial depolarization
- Weitere Details:
- TMRM accumulates and fluoresces orange in polarized mitochondria. Fluorescence drops dramatically on depolarization in apoptotic or metabolically stressed cells. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy., ICT
- Versandbedingungen:
- Blue Ice