6342
Green Live/Dead Stain
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£162.00
- SKU:
- 6342
- Anwendung:
- Flow Cytometry
- Konzentration:
- 500 uM
- Physischer Zustand:
- Cell Culture
- Aufreinigung:
- Green Live/Dead Stain and FLICA® 660 Poly Caspase Assay were used to assess cell death in staurosporine-treated Jurkat cells. Following a four hour induction period, treated cells were stained with the reagents and imaged witha Nikon E800 microscope (DIC overlay image, left). The majority of cells were in the early stages of apoptosis (active caspases stained red with FLICA® 660). One cell in this field of view (bottom right corner) exhibited both red and green fluorescence, indicating the loss of membrane integrity that is characteristic of late-stage apoptosis.
- Lagerbedingungen:
- -20[o]C
- Hersteller:
- ImmunoChemistry Technologies
- Immunogen:
- Dead cells, necrosis
- Weitere Details:
- Green Live/Dead Stain (catalog #6342) is a vital dye that exhibits intact cell membrane exclusion properties analogous to the popular red fluorescing vital dyes, Propidium Iodide (PI), 7-amino- actinomycin D (7-AAD) and DRAQ7™. Like the red fluorescing dyes, Green Live/Dead Stain is excluded from intact, healthy cells due to its polar nature. In the presence of cells exhibiting compromised membrane integrity, Green Live/Dead Stain penetrates the cell and nuclear membrane barriers and intercalates tightly to DNA in a manner analogous to PI and 7-AAD. When bound to DNA, it acquires a greatly enhanced fluorescence potential (>2000X) in the green emission range. These important vital dye properties enable Green Live/Dead Stain to be used in flow cytometry-based protocols to assess the percentage of late apoptotic, necrotic, and membrane-compromised cells within a sample cell population. When bound to nucleic acids, the maximum absorption of Green Live/Dead Stain is 495 nm and the maximum emission is 512 nm (Figure 1). Cells can be viewed through a fluorescence microscope (Figures 2 & 5) or analyzed with a flow cytometer (Figure 4). Green Live/Dead Stain is provided as a 500 uM concentrated stock solution dissolved in DMSO. For flow cytometry applications, a staining concentration of 50 nM is recommended. Therefore, using sample sizes of 0.5 mL, a single 50 uL vial (catalog #6342) provides enough reagent for 1000 tests. For fluorescence microscopy applications, a usage concentration of 0.5 uM is suggested. In this way, a vial is sufficient for 100 tests (0.45 mL sample sizes). Green Live/ Dead Stain can be used with ICT's red FLICA® 660 caspase inhibitor reagents (e.g., catalog #9120) to identify four populations of cells: living; early apoptotic; late apoptotic; and necrotic (Figures 4 & 5)., ICT
- Versandbedingungen:
- Blue Ice