1778
codon-optimized FLP (FLPo) with mCherry Adenovirus (Ad-mCherry-FLPo)
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£654.00
- SKU:
- 1778
- Zusätzliche Namen:
- Ad-mCherry-FLPo|codon-optimized FLP (FLPo) with mCherry Adenovirus
- Konzentration:
- 1E10 PFU/ml
- Lagerbedingungen:
- -70[o]C Avoid freeze/thaw cycles.
- Hersteller:
- Vector Biolabs
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Spezies:
- Virus
- Weitere Details:
- FLP is a site-specific recombinases (SSR) from the I>> integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express both FLPo and mCherry as a fluorescent marker. FLPo and mCherry are driven by the same CMV promoter and separated by a 2A peptide., vectorbiolabs
- Versandbedingungen:
- Dry Ice