1775
codon-optimized FLP Recombinase (FLPo) Adenovirus (Ad-CMV-FLPo)
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£654.00
- SKU:
- 1775
- Zusätzliche Namen:
- Ad-CMV-FLPo|codon-optimized FLP Recombinase (FLPo) Adenovirus
- Konzentration:
- 1E10 PFU/ml
- Lagerbedingungen:
- -70[o]C Avoid freeze/thaw cycles.
- Hersteller:
- Vector Biolabs
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Spezies:
- Virus
- Weitere Details:
- FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the I>> integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This adenovirus express FLPo under a control of a CMV promoter., vectorbiolabs
- Versandbedingungen:
- Dry Ice