XIAP (API3, Baculoviral IAP repeat-containing protein 4, BIRC4, hILP, HILP, IAP3, IAP-like protein, ILP1, Inhibitor of apoptosis protein 3, MIHA, Xiap, X-linked IAP, X-linked inhibitor of apoptosis protein) (HRP)

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£971.00

SKU:: X1034-05E-HRP
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a thirteen amino acid sequence with the C-terminal region of human XIAP.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Weitere Details:: XIAP is an endogenous inhibitor of cell death, which functions by suppressing caspases 3, 7, and 9. The protein is also reported to play a a role in the activation of NF kappa B and the regulation of cyclins and cyclin-dependent kinase inhibitors (CDKIs). Regulators of XIAP activity include XAF-1 and Smac. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: detects a band of ~57kD in human kidney lysates. Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilution determined by the researcher. Recommended Secondary Reagents: Sheep anti rabbit IgG: HRP Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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