W9000-02D
WRN (Werner Syndrome ATP-dependent Helicase, DNA Helicase RecQ-like Type 3, RecQ3, RECQL3, Exonuclease WRN, RecQ Protein-like 2, RECQL2)
Größe
£435.00
- SKU:
- W9000-02D
- Anwendung:
- WB
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to full length human WRN.
- Klon:
- 11C1094(8H3)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Weitere Details:
- The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes including double strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members results in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). WRN is a member of the RecQ family that has been identified as the gene underlying Werner’s syndrome; an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner’s Syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice