V5000-01H
VSV-G Epitope Tag (Vesicular Stomatitis Virus Glycoprotein)
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£735.00
- SKU:
- V5000-01H
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
- Weitere Details:
- VSV is a member of the Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G). G is a type I membrane glycoprotein and is responsible for viral binding to cell surface receptors and initiating the infectious process. VSV-G or vesicular stomatitis virus glycoprotein is found within the pseudo lentiviral cloning vector pHCMV-VSV-G. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:8000-1:32,000 Immunofluorescence: use paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1:200. At higher concentrations, a filamentous background staining may be present. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice