V2110-20M
Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3, Fms-like Tyrosine Kinase 4, Flt4, FLT-4, Tyrosine-protein Kinase Receptor FLT4) (APC)
Größe
£825.00
- SKU:
- V2110-20M
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- 4°C Do not freeze
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Tyr25-Asp770 from the extracellular domain of mouse Vascular Endothelial Growth Factor Receptor 3 (VEGFR-3), expressed in Sf21 cells.
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- VEGFR-3 is a receptor tyrosine kinase that is widely expressed in the early embryo, but is restricted to lymphatic endothelia at later stages of development. Both VEGF C and VEGF D have been shown to bind and activate VEGFR-3. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using bEND.3 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of V2110-20M to up to 1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled goat IgG antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice