V2024-02L

VAMP 2, Recombinant, Human (Synaptobrevin 2, Vehicle-associated membrane)

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£448.00

SKU:: V2024-02L
Molekulargewicht:: 13.8
Reinheit:: ≥95% by SDS PAGE
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM EDTA
Weitere Details:: Synaptobrevin 2 (Vehicle-associated membrane, VAMP2), which is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. Recombinant protein corresponding to aa1-89 of human VAMP 2, His- tag expressed in E. coli (NP_055047). Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG ASQFETSAAK LKRKYW Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20°C. Aliquots are stable for 6 month after receipts at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.