Urokinase Plasminogen Activator Receptor (uPAR, CD87) (Biotin)

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£965.00

SKU:: U2610-04C
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: NS0-derived recombinant human PAR.
Format:: Supplied as a lyophilized powder from PBS and BSA. LAbeled with Bioin. Reconstitute with 0.1% BSA in TBS.
Weitere Details:: Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/mL. The detection limit is~20 ng/lane under non-reducing and reducing conditions. ELISA: coat plate with 100uL/well of the capture antibody, in combination with 100uL/well of thedetection antibody at 200 ng/mL, an ELISA for sample volumes of 100il can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 2 ng/mL. In this format, less than 0.1% cross-reactivity with rmuPAR and rhuPA-1 is observed. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.