U1001-01A-ML550
Ubiquitin Conjugating Enzyme (E2N, UBE2N, Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin-conjugating enzyme, BLU) (MaxLight 550)
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£869.00
- SKU:
- U1001-01A-ML550
- Reinheit:
- Purified by Ion Exchange chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15aa located near the human UBC13 carboxy-terminus.
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Weitere Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Ubiquitin-conjugating enzyme E2N (UBC13) is a 16kD enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation. Applications: Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice