T9300-02
TWIST1 (Twist Homolog 1 (Drosophila), ACS3, bHLHa38, BPES2, BPES3, H-twist, SCS, Twist-related Protein 1)
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£448.00
- SKU:
- T9300-02
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant sequence from the C-terminal region of human TWIST1.
- Klon:
- 8C345
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
- Weitere Details:
- Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. Applications: Suitable for use in ELISA, Western Blot, Dot Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2-2ug/ml Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with T9300-02. Flow Cytometry: 0.5-2ug. HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with T9300-02 or isotype control followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice