T9230-07-ML550
Tyrosinase (TYR, LB24 AB, LB24-AB, Monophenol Monooxygenase, Oculocutaneous Albinism IA, OCA1A, OCAIA, SK29 AB, SK29-AB, Tumor Rejection Antigen AB) (MaxLight 550)
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£965.00
- SKU:
- T9230-07-ML550
- Reinheit:
- Purified by Protein A chromatography
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant tyrosinase protein.
- Klon:
- T311
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
- Weitere Details:
- Tyrosinase is the principal enzyme required for the synthesis of melanin pigment by melanocytes in mammals. It is also a marker of melanocyte differentiation, and is commonly expressed by malignant melanoma. Tyrosinase immunoreactivity studies in formalin-fixed, paraffin-embedded tissues have demonstrated that the T311 antibody is a specific, sensitive marker for the detection of melanocytic lesions. Immunoreactivity has also been shown to correlate inversely with clinical stage of melanoma progression. Because tyrosinase is recognized by cytotoxic T cells from melanoma patients, it has been proposed as an immunotherapeutic target for melanoma vaccines. The molecular mass of tyrosinase is 60-75kD. However, the processed, glycosylated forms of the protein found in melanosomes have been reported at 70-75kD. The T311 antibody identifies a cluster of proteins at 70-80kD, consistent with previous reports. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: 1ug/ml Western Blot: 1-5ug/ml Note: Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, prior to staining, is recommended for optimal immunohistochemical results with paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice