T8199-30A-BIOTIN

Transferrin Receptor (CD71, TR, Tfr, TfnrR) (Biotin)

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£837.00

SKU:: T8199-30A-BIOTIN
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Mouse Concanavilin A activated spleen cells. Epitope: Extracellular
Klon:: 3H3074 (YTA74.4)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Weitere Details:: Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by researcher. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (mouse absorbed) F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Fusion Partners: Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line. Recommended Negative Controls: Rat IgG2a Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.