T8180-04

TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand)

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£435.00

SKU:: T8180-04
Reinheit:: Purified by Protein A affinity chromatography from ascites.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human TRAIL derived from E. coli.
Klon:: 4i170
Format:: Supplied as a lyophilized powder from PBS. Reconstitute with 500ul of sterile ddH2O.
Weitere Details:: Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml (100ul/well) detects at least 500pg/ml of recombinant human TRAIL when used with biotinylated anti-human TRAIL (catalog# 143686) as detection antibody at a concentration of 1-2ug/ml. Western Blot: 0.25-0.5ug/ml. The detection limit is 0.25-0.5ng/lane of recombinant human TRAIL, under either reducing or non-reducing conditions. Neutralization: 0.3-0.6ug/ml to yield one-half the maximal inhibition of the biological activity of human TRAIL (10ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.