Toso, CT (Regulator of Fas-induced Apoptosis, Fas Apoptotic Inhibitory Molecule 3, FAIM3) (MaxLight 650)

Größe

£947.00

SKU:: T8067-50C-ML650
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: A 13aa peptide from near the carboxy terminus of human Toso.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Weitere Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and hematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kD in SDS PAGE. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects a band of ~43kD in human lung tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice