T5585-25A
TIMP2 (Metalloproteinase Inhibitor 2, CSC-21K, Tissue Inhibitor of Metalloproteinases 2, TIMP-2)
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£761.00
- SKU:
- T5585-25A
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Protein corrresponding to aa56-66 (DSGNDIYGNPI) from human TIMP2.
- Klon:
- 3A4
- Format:
- Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide. For antibody without BSA and azide see T5585-25AX.
- Weitere Details:
- TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 innibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT. Requires HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 1-2ug/ml Immunofluorescence: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice