Thrombomodulin (CD141, TM, Fetomodulin) (Biotin)

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£876.00

SKU:: T5048-10
Reinheit:: Purified by Immunoaffinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to aa17-517 from mouse Thrombomodulin extracellular domain expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3 (20mM Tris, 150mM sodium chloride) 0.1% BSA.
Weitere Details:: Thrombomodulin (TM; also fetomodulin and CD141) is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant. Mouse Thrombomodulin/TM is 577 amino acids (aa) in length. It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480). Thrombin binds to aa403-480. One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577). Over aa17-517, mouse TM is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 50ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume ≤200ul. Tested on bEnd.3 cells. Western Blot: 0.1-0.2ug/ml; The detection limit is ~0.5ng/lane and 25ng/lane under non-reducing and reducing conditions, respectively. Shows less than 1% crossreactivity with recombinant human Thrombomodulin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after reciept at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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