T2550-01B

Tenascin C (Tenascin-C, TNC, TN-C, TN, Cytotactin, Glioma-associated Extracellular Matrix Antigen, GMEM, GP-150-225, Hexabrachion, HXB, JI, Myotendinous Antigen, Neuronectin)

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£453.00

SKU:: T2550-01B
Reinheit:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Purified protein from human mammary tumor.
Klon:: T2H5
Format:: Supplied as a liquid in TBS, 0.09% sodium azide.
Weitere Details:: Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:2000, suitable for use in direct ELISA applications, using T2550-01H as a standard. Immunohistochemistry (Paraffin/frozen): Staining of paraffin sections may be enhanced using trypsin digestion or heat mediated antigen retrieval using sodium citrate buffer pH 6.0. Optimal dilutions to be determined by the researcher. Positive Control: Human tonsil Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice