T-box 6 (Tbx6, T-box Family of Transcription Factors)

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£908.00

SKU:: T1848-30
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant corresponding to aa1-280 of human T-box expressed in E. coli (O95947). Species Sequence Homology: Canine (94%), mouse (90%)
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: TBX6 (T-box protein 6) is a 47kD member of the T-box family of transcriptional regulators. It is expressed during embryogenesis, plays a role in presomitic mesoderm development, and promotes delta-like 1 transcription. Human TBX6 is 436 amino acids (aa) in length. It contains one T-box DNA-binding domain (aa90-277). There is one alternate splice form that shows a 15 aa substitution for aa 281-436. Over aa 1-280, human TBX6 shows 94% and 90% aa identity with canine and mouse TBX6, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry (fluorescence): 10ug/ml detected T-box 6 in cryosections of E10.5 mouse embryos. Tissue was fixed overnight in buffered zinc formalin fixative at 4ºC. Sections were blocked with 10% donkey serum, 0.1% Triton in 1% BSA in PBS for 45 minutes at RT. After blocking sections were incubated with diluted T1848-30 for 3 hours at RT folled by a Dk xGt IgG (NorthernLights 557) at RT for 1 hour. Between each step, sections were washed with 0.1% BSA, PBS. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice