T1037-BIOTIN
Tau1 (Tau Protein1, Microtubule-associated Protein, MAP) (Biotin)
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£1,196.00
- SKU:
- T1037-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified denatured bovine microtubule associated proteins
- Klon:
- 2Q123 (PC1C6)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Weitere Details:
- Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: Bovine brain microtubule proteins purified by two cycles of assembly and disassembly (9) are dissolved in SDS-PAGE sample buffer. 5ug of the microtuble preparation per lane is loaded onto a 4% to 20% SDS-PAGE gradient gel along side molecular weight markers (14.3-200kD). After separation by electrophoresis, the proteins are blotted onto nitrocellulose. Tau is detected as a series of 5 bands (52–68kD). Immunohistochemistry: Stains axons in tissue primarily, however in culture Tau expression is not restricted to just axons. Dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimer’s brain tissue with T1037. This treatment changes the staining pattern of T1037 to include cell bodies, dendrites and axons of neurons. In untreated samples, T1037 stains axons only. Optimal dilutions to be determined by the researcher. Positive Control: IMR5 cells, Human T98G glioblastoma cells or Alzheimer’s disease brain tissue Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice