S9100-01T
SYK (Spleen Tyrosine Kinase, HGNC:11491, p72Syk, Tyrosine Protein Kinase SYK)
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£550.00
- SKU:
- S9100-01T
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa5-360 of human Syk.
- Klon:
- SYK-01
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
- Weitere Details:
- Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml mmunohistochemistry (paraffin sections): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice