S7972-15F-ML490
STAT 5b (Signal Transducer and Activator of Transcription 5b) (MaxLight 490)
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£1,023.00
- SKU:
- S7972-15F-ML490
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- aa774-787 of human STAT5B (CGRPMDSQWIPHAQS)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Weitere Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: detects STAT5B (doublet of 90kD and 92kD) in human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: immunoprecipitates STAT5B from A431 carcinoma cells and a previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice