STAT 1, CT (Signal Transducer and Activator of Transcription 1) (Biotin)

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£1,023.00

SKU:: S7969-05-BIOTIN
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: GST fusion-protein containing the carboxy terminal 36aa of human STAT1a. This sequence is missing in the b isoform.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Weitere Details:: Positive Antigen Control: Jurkat cell lysate. Use 20ug per lane for minigels. Applications: Suitable for use in Western Blotting, Immunoprecipitation. Western Blotting: 0.5–2ug/ml detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4mg immunoprecipitated STAT1 from 500mg of human Jurkat RIPA lysate. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage , store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.