S5620-02D
Sprouty 2 (SPRY2, Protein Sprouty Homolog 2, Spry-2)
Größe
£837.00
- SKU:
- S5620-02D
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Met1-Ala175 from human SPRY2.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- SPRY2 (sprouty homolog 2) is a 35kD member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC-delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 aa in length and contains one CBL-TKB binding site (aa53-59) that is phosphorylated at Tyr55, a Ser-rich region (aa125-131), a Cys-rich domain (aa178-301) and a PxxPxR motif that mediates ERK inhibitory activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using SH‑SY5Y human neuroblastoma cell line, PC‑3 human prostate cancer cell line, and C2C12 mouse myoblast cell line as samples. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice