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S1013-87Q-ML490

SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (MaxLight 490)

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£960.00

SKU:
S1013-87Q-ML490
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:
4°C Do Not Freeze
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Rat peritoneal macrophages
Klon:
OX-41
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Weitere Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Optimal dilution determined by the researcher Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed) Immunohistochemistry: Recommended Secondary Reagents: Goat anti mouse IgG:HRP (rat adsorbed) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice