S1013-58B
Siglec 3 (Sialic Acid-binding Immunoglobulin-like Lectin 3) (PE)
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£722.00
- SKU:
- S1013-58B
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- 4°C Do not freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to the extracellular domain of human Siglec‑3/CD33.
- Klon:
- 7H35
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- Siglecs are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal, Ig-like V-type domain that mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (2 to17), a single transmembrane region, and a cytoplasmic tail. The siglecs can be broadly classified into two subgroups: siglecs-1, -2, and -4, and a siglec-3/CD33-related subgroup (siglecs-3, and -5 through -13 in primates) defined by sequence similarity and clustered gene localization. They are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Siglec-4/MAG is a myelin component in Schwann cells and oligodendrocytes. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Although siglec functions continue to be defined, most have intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM), implicating them in the suppression of immunoreceptor signaling. They may also participate in cell/cell interactions or act as receptors for the entry of viral or bacterial pathogens. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice