S1000-77J
SERPINB6 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 6, Placental Thrombin Inhibitor, (Proteinase Inhibitor 6, PI6) (Biotin)
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£863.00
- SKU:
- S1000-77J
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Sf 21-derived rhSerpin B6. (aa 2 - 376)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- ine protease inhibitor-clade B#6; also placental thrombin inhibitor) is a 42kD, cytosolic member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types (keratinocytes; monocytes; mast cells), and shows inhibitory activity towards thrombin, plasmin and chymotrypsin. Human Serpin B6 is 376 amino acids (aa) in length. An active inhibitor site lies between Glu327 and Met338. Multiple potential isoforms exist. One shows a 28 aa substitution for the N-terminal 191 amino acids. Three others have alternate start sites that vary from 14-33 aa’s upstream of the standard start site. Human Serpin B6 shares 76% aa identity with mouse Serpin B6. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml detection limit for rhSerpin B6 is ~ 5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice