Semaphorin 3E (Semaphorin-3E, SEMA3E, Collapsin-5, Coll5, Coll-5, KIAA0331, MSEMAH, Msema-H, M-Sema-H, M-SEMAH, M-SemaK, Sema Domain Immunoglobulin Domain (Ig), Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted 3E, SEMAH)

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£869.00

SKU:: S0668-50A
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Thr25-Ser775 (Arg557Ala and Arg560Ala) from human Semaphorin 3E expressed in NSO cell line.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: Semaphorin 3E (previously SemaH) is a secreted protein belonging to the semaphorin family. Semaphorin 3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Like other Semaphorin 3 proteins, the 775 aa human Semaphorin 3E contains a signal sequence, a Semaphorin domain, a C2-like Ig domain and a C-terminal basic domain. Disulfide-linked dimerization and furin-type proteolytic processing increase Semaphorin 3E bioactivity. Mature human and mouse Semaphorin 3E share 90% aa identity. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 5ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10ug/ml to detect Semaphorin 3E on MDA-MB-453 human breast cancer cells. Immunohistochemistry: 10ug/ml to detect Semaphorin 3E in cryosections of E13.5 mouse embryos. Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Semaphorin 3E. The detection limit for rhSemaphorin 3E is approximately 1.0ng/well. In this format, this antibody shows approximately 70% cross-reactivity with rmSemaphorin 3E and less than 5% cross-reactivity with rmSemaphorin 3C and rmSemaphorin 3F. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice