Secretory Component (Not for Export EU)

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£466.00

SKU:: S0630-01J
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Purified free secretory component isolated from pooled rhesus monkey milk
Format:: Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Weitere Details:: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Applications: Suitable for use in ELISA, Immunoelectrophoresis, Immunoprecipitation and Single and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 2ul against 120ul antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice