Succinate Dehydrogenase Complex Assembly Factor 1, SDHAF1

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£642.00

SKU:: S0553-27
Reinheit:: Purified by peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-HDSTGAPETRPDGR, from the C-Terminus of human SDHAF1 (NP_001036096.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.3-1ug/ml detects ~19kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 12.8kD according to NP_001036096.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.