S0550-08
ST6GALNAC5 (Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, GD1 alpha Synthase, GalNAc alpha-2,6-sialyltransferase V, ST6GalNAc V, ST6GalNAcV, Sialyltransferase 7E, SIAT7-E, SIAT7E)
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£800.00
- SKU:
- S0550-08
- Anwendung:
- ICC
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa30-336 from human ST6GALNAC5 expressed in CHO cell line.
- Klon:
- 12K170
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues. They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases. ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAca2,3GalB1,3GalNAcB1,4(NeuAca2, 3)GalB1,4GlcB1Cer) from GM1b (NeuAca2,3GalB1,3GalNAcB1,4GalB1,4GlcB1Cer), and its expression is restricted to the brain. ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier. The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method suggesting the substrate specificity of ST6GALNAC5 may require further characterization. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice