Sam 68 (KH domain-containing, RNA-binding, signal transduction-associated protein 1, p68, GAP-associated tyrosine phosphoprotein p62) (Not for Export EU)

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£767.00

SKU:: S0070-86
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated peptide corresponding to amino acid residues 331-348 of human Sam 68. Species Sequence Homology: rat
Format:: Supplied as a liquid. 0.05% sodium azide.
Weitere Details:: KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1, p68, p21 Ras GTPase-activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the “signal transduction and activation of RNA” (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detected Sam68 in 10ug of MDA-MB-468 cell lysate. Immunohistochemistry: 1:600 detected Sam 68 in mouse colon tissue Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice