S0052-17H1

S100A8 (Calgranulin A, Calcium-binding Protein A8, CP-10, MRP8, 60B8AG, CAGA, CFAG, CGLA, L1Ag, MA387, MIF, NIF, P8)

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£863.00

SKU:: S0052-17H1
Reinheit:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Met1-Glu89 (predicted) from mouse S100A8, expressed in E. coli.
Klon:: 8H150
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10kD member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal a-helix, followed by a calcium-binding EF-hand segment, and a C-terminal a-helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 is 80% and 57% aa identical to rat and human S100A8, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using mouse leukocyte Immunocytochemistry: 8-25ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-74F: IgG, Murine X-Adsorbed (FITC) Pab Gt x Rt Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice