RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34)

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£793.00

SKU:: R3400-02M
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Cocktail of peptides corresponding to the N-terminus of human and mouse p32.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: The p32 protein is a doughnut-shaped trimer that is primarily localized in the mitochondria. It has also been reported to be present at the cell surface and in the nucleus. The role of p32 in mammalian cells is unclear. The yeast p32 homologue has been reported to regulate oxidative phosphorylation, and a link to autophagy has been proposed. Elevated expression of p32 in cancer has also been noted. Additionally, p32 is primarily localized in hypoxic/nutrient-deprived regions within tumors and that, in addition to tumor cells, a tumor-associated macrophage/myeloid cell subpopulation closely linked to tumor lymphatics expresses high levels of p32. The expression of p32 at the cell surface, where it can be targeted with p32-binding antibodies and peptides, adds a second level of tumor specificity to p32 expression. The unique expression pattern of p32 in tumor cells, tumor lymphatics, and tumor-associated macrophages/myeloid cells makes p32 a potential target for the diagnosis and therapy of cancer. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 5ug/ml detects p32 on 10ug of A431 cell lysate. Immunocytochemistry: 1:500 detects p32 in HeLa and A431 cells. Optimal dilutions to be determined by the researcher. Positive Control: A431 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice