R2998-26C
ROK alpha/ROCK-II (Rho-associated protein kinase 1 / Rho-associated coiled-coil containing protein kinase 2)
Größe
£825.00
- SKU:
- R2998-26C
- Reinheit:
- Supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- His-tagged fusion protein corresponding to residues 2-543 of rat ROKa/ROCK-II. Cellular Localization: Cytoplasm. Centrosome › centriole. Species sequence homology: mouse and bovine.
- Klon:
- A9W4
- Format:
- Supplied as a liquid in 0.05% sodium azide.
- Weitere Details:
- Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKa/ROCK-II and ROKb/ROCK-I. These large cellular proteins (150-160kD), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:5000 detected ROKα/ROCK-II in RIPA lysates from PC-12 cells. Immunoprecipitation: 4ul immunoprecipitated ROKα/ROCK-II from 500ug of PC-12 lysate. Optimal dilutions to be determined by the researcher. Positive Control: RIPA lysates from PC-12 cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice