Ro/SSA

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£429.00

SKU:: R2997
Reinheit:: Purified by fractionation of delipidated and defibrinated donor human serum.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Human
Immunogen:: This product is Ro/SSA autoantibody derived from human serum.
Format:: Supplied as a lyophilized powder from PBS (0.01M phosphate/0.13M sodium chloride), pH 7.2, 0.02% sodium azide. Reconstitute with 2ml ddH2O.
Weitere Details:: The Ro/SSA antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the Ro/SSA antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-Ro/SSA autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the Ro/SSA antigen may be used as a diagnostic aid. Elevated levels of Anti-Ro/SSA have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-Ro/SSA and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with Ro/SSA precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-Ro/SSA have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers. Applications: Suitable for use in ELISA and Immunodiffusion. Other applications not tested. Recommended Dilutions: ELISA: 1:100 Optimal dilutions to be determined by the researcher. Titer: Immunodiffusion: 1:4 Ro/SSA Autoantibody Activity (by ELISA): 6447EU/ml (lot-specific; value may differ from lot to lot). Other Autoantibody Activity in the Serum (by ELISA): SSB: 3.34EU/ml SCL: 3.17EU/ml Sm: 2.55EU/ml Sm/RNP: 2.74EU/ml Jo-1: 2.97EU/ml Interpretation of ELISA Results: Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice