R2031-74G-PE
RIP3 (Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3, Receptor-interacting Protein 3, RIP-3, mRIP3) (PE)
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£869.00
- SKU:
- R2031-74G-PE
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 14aa near the C-terminus of mouse RIP3. Epitope: within the last 50aa
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues. Applications: Suitable for use in FLISA, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: detects a band of ~56kD in Mouse 3T3 whole cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice