R2031-62G

Rictor (Rapamycin Insensitive Companion of mTOR, Rapamycin-insensitive Companion of mTOR, RICTR, AVO3, KIAA1999, Likely Ortholog of Mouse TORC2 Specific Protein AVO3 (S. cerevisiae), mAVO3, Pianissimo)

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£748.00

SKU:: R2031-62G
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Rictor.
Klon:: 10F659 (9F1.2)
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/g/uL. mTORC2 is activated by growth factors and other mitogenic signals. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects Rictor on 10ug of HEK293 cell lysates Immunoprecipitation: 1ug/ml detects Rictor from 100ug of HEK293 cell lysates Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cell lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.