R1691-97
Retinoic Acid Receptor Responder 1 (RARRES1, Tig1)
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£811.00
- SKU:
- R1691-97
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Pro42-Phe283 of mouse RARRES1 expressed in E. coli. Species Sequence Homology: rat (88%), human (79%).
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- RARRES1 (Retinoic Acid Receptor Responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Mouse RARRES1 is 349 amino acids (aa) in length. Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa75-95. One potential 350aa splice variant exists that shows an almost complete aa divergence after Pro105. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Fluorescence): 5-15ug/ml; Detects RARRES1 in cryosections of mouse liver. Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4°C. Sections were blocked with 10% donkey serum and 0.1% Triton X-100 in PBS, 1% BSA for 45 minutes at RT. After blocking, sections were incubated with R1691-97 for 3 hours at RT, followed by donkey anti-goat IgG (NL557) for 1 hour at RT. Between each step, cells were washed with PBS, 0.1% BSA. Western Blot: 1ug/ml mouse spleen tissue lysates Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice