Resistin-like Molecule, alpha (RELMa)

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£576.00

SKU:: R1587-04A
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant mouse RELM alpha, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Weitere Details:: Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml (using 100µl/well antibody solution) allows the detection of 0.2-0.4ng/well of recombinant mouse RELM alpha. Western Blot: 0.1-2ug/ml. The detection limit for recombinant mouse RELM alpha is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 4ng/ml. Incubate overnight at 4ºC. Heat induced antigen retrieval using 100mM citric acid is recommended. Stained 4% PFA, paraffin-embedded sections of mouse cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.