R1495-03C-HRP
RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae), Up-frameshift Mutation 1 Homolog (S. cerevisiae), Up Frameshift Suppressor 1 Homolog, UPF1 Regulator of Nonsense Transcripts Homolog, Yeast Upf1p Homolog) (HRP)
Größe
£1,023.00
- SKU:
- R1495-03C-HRP
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic linear peptide corresponding to RENT1. Species sequence homology: mouse, rat, bovine and canine.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. RENT1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: U2OS cell lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice