R1171-01K
Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303)
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£761.00
- SKU:
- R1171-01K
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Raptor.
- Klon:
- 10F657 (1H6.2)
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected Raptor on 10ug of HEK293 cell lysate. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with R1171-01K. Proteins were visualized using a goat anti-mouse IgG (HRP) and chemiluminescence detection system. Immunoprecipitation: 20ug Immunocytochemisty: 1: 500 detected Raptor in NIH/3T3 cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Positive Control: HEK 293 cell lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice