Rad50 (DNA Repair Protein RAD50, hRad50, hsRAD50, Mrell, Nbs1 Complex, RAD50-2, RAD50 Homolog (S cerevisiae), RAD50 Homolog, RAD50 PEN, Rad50l, Truncated RAD50 Protein) (Not for Export EU)

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£594.00

SKU:: R0450-01J
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Human Rad50 zinc hook region (aa628-787) expressed in E. coli. (Q92878). Cellular Localization: Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
Format:: Supplied as a liquid. No preservatives added.
Weitere Details:: RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 1-5ul Western Blot: 1:1000-1:10,000 detects a band at ~153kD Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice