P9544-45
Pyruvate Dehydrogenase
Größe
£581.00
- SKU:
- P9544-45
- Reinheit:
- Purified by peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the sequences of both alpha1 and alpha12 subunits of human pyruvate dehydrogenase.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Weitere Details:
- Pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO 2 in the presence of NAD+ (1). Acetyl-CoA then goes into citric acid cycle to react with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis (1). The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and citric acid cycle and lipid metabolism (1). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two types of subunits: alpha and beta. This enzyme catalyzes the removal of one carbon as CO 2 from pyruvate. Mutations in the alpha subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice