P9182-16M

PTEN Tumor Suppressor Protein (Phosphatase and Tensin Homolog, Bannayan Zonana Syndrome, BZS, Multiple Hamartoma (Cowden Syndrome), MHAM, Mutated in Mutiple Advanced Cancers 1, MMAC1, Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and Dual-specificity Protein Phosphatase PTEN, Platelet Antigen BR, PTEN1, Tensin Homolog, TEP1, VLA 2 Receptor Alpha Subunit)

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£837.00

SKU:: P9182-16M
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant corresponding to Thr2-Val403 from human PTEN expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: The tumor suppressor gene PTEN encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4). Applications: Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice